Neurosyil (often seen spelled Neurosil / Nucleosil) C4, 300 Å HPLC columns are mainly used for large biomolecules. The key is the combo of short alkyl chain (C4) + large pore size (300 Å).
Here’s what they’re typically used for
Main uses of C4, 300 Å columns
1. Protein analysis (big one)
Intact proteins
Therapeutic proteins (mAbs, insulin, growth hormones)
Protein isoforms and variants
The 300 Å pores let large proteins actually enter the stationary phase instead of getting excluded