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HPLC Sorbent

In High-Performance Liquid Chromatography (HPLC), sorbents (also called stationary phases) are the materials packed into the column that interact with analytes and enable their separation. Their uses depend on their chemistry, particle size, porosity, and surface functionalization.

HPLC sorbents are primarily used to separate complex mixtures based on interactions like:

  • Hydrophobicity (reverse-phase C18, C8 sorbents)
  • Polarity (normal-phase silica)
  • Charge interactions (ion-exchange sorbents)
  • Size (size-exclusion chromatography)
  • HPLC sorbents help separate:
  • Pesticides
  • Toxins
  • Pollutants
  • Additives and preservatives
  • Vitamins, amino acids, sugars

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HPLC Sorbent

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